Biology

Core Practical 5

(i) use a light microscope to make observations and labelled drawings of suitable animal cells (ii) use a graticule with a microscope to make measurements and understand the concept of scale MARKING SCHEME More RECOMMENDED ADDITIONAL PRACTICAL​ Investigate factors affecting the growth of pollen tubes.

Core Practical 4

Investigate the effect of temperature, pH, enzyme concentration and substrate concentration on the initial rate of enzyme-catalysed reactions. MARKING SCHEME

Core Practical 3

Investigate membrane properties including the effect of alcohol andtemperature on membrane permeability. MARKING SCHEME More RECOMMENDED ADDITIONAL PRACTICAL 1​ Investigate tissue water potentials using plant tissue and graded concentrations of a solute. RECOMMENDED ADDITIONAL PRACTICAL 2 Use a semi-quantitative method to estimate protein concentration using biuret reagent and colour standards.

Core Practical 1

Use a semi-quantitative method with Benedict’s reagent to estimate the concentrations of reducing sugars and with iodine solution to estimate the concentrations of starch, using color standards. MARKING SCHEME More RECOMMENDED ADDITIONAL PRACTICAL​ Investigate the structure of a mammalian heart by dissection.